Yeast Nitrogen Base without Amino Acids and without Phosphate, supplemented with KCl Size:250g is omitted from this formulation

is omitted from this formulation of Yeast Nitrogen base without Amino acids to provide the researcher the possibility to grow yeast cells in the absence of Iron or to replace this element

1 grams of Neidhardt supplement mixture in one litre of distilled water

C6H12O6  180

This formulation supports the growth of most strains that are capable of growing on HL5

L-Broth is a nutritionally rich medium originally developed for growth and maintenance of recombinant E

Yeast Nitrogen Base without Amino Acids and without Phosphate, supplemented with KCl Size:250g is omitted from this formulationThe absence of phosphate (KH2PO4) provides the possibility to include alternative sources of phosphate, like P32 labeled components, or to select an optimal concentration of phosphate for certain yeast cell lines. By omitting Potassium DiHydrogen Phosphate (KH2PO4), not only Phosphate (PO4 3) but also Potassium (K ) is omitted. As a result yeasts are not only limited in Phosphate, but also in Potassium. This might cause problems in osmo molarity and